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Fluo-8, AM鈣離子熒光探針【CAS 1345980-40-6】
NMR COA MSDS - Names：
CalciFluor Fluo-8, AM
- CAS號：
1345980-40-6
MDL Number： - MF(分子式)： C50H50N2O23 MW(分子量)： 1046.93
- EINECS: Reaxys Number:
- Pubchem ID:54575324 Brand:BIOFOUNT
Fluo-8, AM鈣離子熒光探針;(CalciFluor™ Fluo-8, AM) 是一種綠色熒光鈣結合染料，是一種優于傳統 Fluo-4, AM 的 Ca2+ 熒光探針。熒光 : λex 494 nm, λem 517 nm in DMSO
| 貨品編碼 | 規格 | 純度 | 價格 (￥) | 現價(￥) | 特價(￥) | 庫存描述 | 數量 | 總計 (￥) |
|---|---|---|---|---|---|---|---|---|
| HCM0029B-1mg | 1mg | 98%ab | ￥ 0.00 | ￥ 0.00 | 4800 | 2-3weeks | ￥ 0.00 |
| 中文別名 | 鈣離子熒光探針Fluo-8, AM;鈣離子熒光探針；鈣熒光探針Fluo-8 AM；鈣離子探針；鈣熒光探針;鈣熒光探針 Fluo-8 AM |
| 英文別名 | CalciFluor Fluo-8, AM；Fluo-8, AM；Fluo-8 AM；Bis(acetoxymethyl) 2,2'-((4-(6-(acetoxymethoxy)-3-oxo-3H-xanthen-9-yl)-2-(2-(bis(2-acetoxymethoxy)-2-oxoethyl)amino)phenoxy)ethoxy)phenyl)azanediyl)diacetate |
| CAS號 | 1345980-40-6 |
| Inchi | InChI=1S/C50H50N2O23/c1-30(53)65-25-70-37-12-14-39-44(20-37)75-43-19-36(58)11-13-38(43)50(39)35-10-15-41(52(23-48(61)73-28-68-33(4)56)24-49(62)74-29-69-34(5)57)45(18-35)64-17-16-63-42-9-7-6-8-40(42)51(21-46(59)71-26-66-31(2)54)22-47(60)72-27-67-32(3)55/h6-15,18-20H,16-17,21-29H2,1-5H3 |
| InchiKey | BKYHTWFRZPRHNM-UHFFFAOYSA-N |
| 分子式 Formula | C50H50N2O23 |
| 分子量 Molecular Weight | 1046.93 |
| 溶解度Solubility | Soluble in DMSO, and water (low). |
| 性狀 | Solid power |
| 儲藏條件 Storage conditions | Desiccate at -20° C |
Fluo-8, AM 鈣離子熒光探針CalciFluor™ Fluo-8, AM 的應用
CalciFluor™ Fluo-8, AM 已開發用于改善細胞負載和鈣響應，同時保持便利的 Fluo-3 和 Fluo-4 光譜波長，最大激發 @ ~490 nm 和最大發射 @ ~520 nm。 與 Fluo 4 AM 類似，陰離子羧基被乙酰氧基甲酯保護，允許膜滲透，隨后被細胞內酯酶去除 - 允許 Ca2+ 檢測。
CalciFluor™ Fluo-8, AM has been developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation @ ~490 nm and maximum emission @ ~520 nm. Similarly to Fluo 4 AM, the anionic carboxy groups are protected with acetoxy methyl esters allowing membrane permeability, which are subsequently removed by intracellular esterases - allowing Ca2+ detection. However CalciFluor™ Fluo-8, AM is efficiently loaded into cells at room temperature (whereas Fluo-3 and Fluo-4 require much more harsh loading conditions), and CalciFluor™ Fluo-8, AM is 2 times brighter than Fluo-4 and 4 times brighter than Fluo-3. These features, as well as a lower price, make CalciFluor™ Fluo-8, AM a superior in vivo calcium indicator.
FLUO-8 (AM) 是 Fluo-8 的一種可滲透細胞的乙酰氧基甲基 (AM) 酯衍生物。 亮度大約是 Fluo-4 的兩倍。 吸光度 452 nm (26,000)； 發射 515 nm（AM 水解后；溶于 10 mM EGTA、100 mM KCl、10 mM MOPS，pH 7.2）
Fluo-8 AM, green fluorescent calcium binding dye -- 熒光細胞成像
U2OS 細胞在 96 孔黑壁/透明底部 costar 板中以每 100 µL/孔 40,000 個細胞過夜播種。 去除生長培養基，將細胞與 100 µl 4 µM Fluo-4 AM 或 Fluo-8® AM 在 HHBS 中在 37 °C、5% CO2 培養箱中孵育 1 小時。 細胞用 200 µl HHBS 洗滌兩次，然后使用 FITC 通道在熒光顯微鏡下成像。
| 產品說明 | Fluo-8, AM鈣離子熒光探針;(CalciFluor Fluo-8, AM) 是一種綠色熒光鈣結合染料，是一種優于傳統 Fluo-4, AM 的 Ca2+ 熒光探針。熒光 : λex 494 nm, λem 517 nm in DMSO |
| Introduction | CalciFluor Fluo-8, AM is a green fluorescent calcium binding dye, a superior Ca2+ fluorescent probe than traditional Fluo-4, AM. |
| Application1 | |
| Application2 | |
| Application3 |
Calcium measurements are critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Fluo-3 AM and Fluo-4 AM are most commonly used among the visible light-excitable calcium indicators for live-cell calcium imaging. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis and require harsh cell loading conditions to maximize their cellular calcium responses. Fluo-8® dyes are developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelengths of Ex/Em = ∼490/∼520 nm. Fluo-8® AM can be loaded into cells at room temperature, while Fluo-3 AM and Fluo-4 AM require 37°C for cell loading. In addition, Fluo-8® AM is two times brighter than Fluo-4 AM and four times brighter than Fluo-3 AM. AAT Bioquest offers a set of our outstanding Fluo-8® reagents with different calcium-binding affinities (Fluo-8® Kd = 389 nM; Fluo-8H™ Kd = 232 nM; Fluo-8L™ Kd = 1.86 µM; Fluo-8FF™ Kd = 10 µM). We also offer versatile packing sizes to meet your special needs (e.g., 1 mg, 10x50 µg, 20x50 µg, and HTS packages) with no additional packaging charge.
| 警示圖 | |
| 危險性 | |
| 危險性警示 | No data available |
| 安全聲明 | H319 (100%): Causes serious eye irritation [Warning Serious eye damage/eye irritation] |
| 安全防護 | P264, P280, P305+P351+P338, and P337+P313 |
| 備注 |
| 1. Liu L et al. TRPM7 promotes the epithelial-mesenchymal transition in ovarian cancer through the calcium-related PI3K / AKT oncogenic signaling. J Exp Clin Cancer Res 38:106 (2019). |
| 2. Domínguez A et al. Effect of perfluorodecanoic acid on pig oocyte viability, intracellular calcium levels and gap junction intercellular communication during oocyte maturation in vitro. Toxicol In |
| 3. Bender BJ et al. Structural Model of Ghrelin Bound to its G Protein-Coupled Receptor. Structure 27:537-544.e4 (2019). |
| 4. Datta G et al. HIV-1 gp120 Promotes Lysosomal Exocytosis in Human Schwann Cells. Front Cell Neurosci 13:329 (2019). |
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