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蛋白質(zhì)和肽修飾試劑介紹：
蛋白質(zhì)和肽的修飾試劑是用于修飾氨基酸側(cè)鏈以改變天然電荷、阻斷或暴露反應(yīng)性結(jié)合位點(diǎn)、滅活官能團(tuán)和改變官能團(tuán)以產(chǎn)生交聯(lián)和標(biāo)記目標(biāo)的化學(xué)試劑。

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正確選擇蛋白質(zhì)和肽修飾試劑
1. 胺基蛋白質(zhì)和肽修飾試劑

產(chǎn)品	功能
Sulfo-NHS-Acetate	胺反應(yīng)性（NHS 酯）化合物永久阻斷伯胺（例如，蛋白質(zhì)賴氨酸側(cè)鏈），以防止相互作用或隨后的結(jié)合。
Citraconic anhydride	胺反應(yīng)性化合物 (CAS 616-02-4) 可逆地用羧基封端賴氨酸和其他伯胺； 在 pH 7 穩(wěn)定，但在 pH 3 至 4 時(shí)水解（解偶聯(lián)）。
TFCS	胺反應(yīng)性（NHS 酯）化合物； 末端的三氟乙?；梢陨院笕コ员┞冻鲂碌牟?。

 

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2. 異雙官能團(tuán)蛋白質(zhì)和肽修飾試劑

產(chǎn)品	產(chǎn)品功能
SATP	Short (4.1A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.
SAT(PEG)4	Long (18.2A), PEGylated form of SATA, an amine-reactive (NHS ester) sulfhydryl-addition reagent; contains 4-unit polyethylene glycol spacer arm.
SATA	SATA is a short-chain (2.8 angstrom spacer arm) reagent for covalent modification of primary amines and addition of a protected yet exposable sulfhydryl group, enabling heterobifunctional crosslinking strategies.
EMCA	EMCA is a sulfhydryl-reactive (maleimide) compound to permanently cap protein or peptide cysteines with carboxyl groups (make caproic acid derivatives).
AEDP	AEDP is an amine-and-carboxyl group containing compound that contains an internal disulfide bond; use with crosslinkers to modify functional groups on proteins and surfaces.
Traut's Reagent	2-Iminothiolane-HCl (CAS 4781-83-3) is an amine-reactive thiolation reagent to cap protein primary amines with sulfhydryl groups for immediate crosslinking.

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3. 巰基蛋白質(zhì)和肽修飾試劑

產(chǎn)品	產(chǎn)品功能
NEM	N-Ethylmalemide (NEM) is a small, sulfhydryl-reactive (maleimide) compound to permanently block the reduced cysteines of proteins or peptides to prevent disulfide bond formation.
MMTS	Methyl methanethiosulfonate (MMTS) (CAS 2949-92-0) reversibly block cysteines (sulfhydryl groups) to help facilitate the study of enzyme activation and protein function.
EMCA	EMCA is a sulfhydryl-reactive (maleimide) compound used to permanently cap protein or peptide cysteines with carboxyl groups (making caproic acid derivatives).

 

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4. 其他產(chǎn)品

產(chǎn)品	產(chǎn)品功能
APTS	3-Aminopropyltriethoxysilane (APTS)is a silylation reagent for coating glass and silica surfaces to add primary amines, which can be used to crosslink and immobilize proteins and other molecules.
NaIO4	Sodium meta-periodate (NaIO4) (CAS 7790-28-5) is used to gently oxidize glycoprotein carbohydrate sugars to create reactive aldehydes (carbonyls) for crosslinking and immobilization.
IAA	Iodoacetic acid (IAA) is C2H3IO2 (CAS 64-69-7) is used to S-carboxymethylate sulfhydryls (reduced cysteines) at slightly alkaline pH for use in various protein biology methods.
Iodoacetamide	2-Iodoacetamide (CAS 144-48-9) is a sulfhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterization and peptide mapping. Alkylation after cystine reduction results in the covalent addition of a carbamidomethyl group (57.07 Da) and prevents the formation of disulfide bonds.
Chloroacetamide	2-Chloroacetamide (CAS 79-07-2) is a sulfhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterization. Alkylation with chloroacetamide after cysteine reduction results in the covalent addition of a carbamidomethyl group (57.07 Da) and prevents the formation of disulfide bonds.
Hydroxylamine-HCl	Hydroxylamine-HCl (NbOH.HCl) (CAS 7803-49-8) is used to cleave Asn-Gly peptide bonds or to deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent.
GlycoLink Coupling Catalyst	Aniline (CAS 62-53-3) and an optimized buffer is used to enhance aldehyde-hydrazide crosslinking efficiency for glycoprotein immobilization and conjugation.